Construct: shRNA TRCN0000061892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019020.2-224s1c1
DNA Barcode:
GATCATCTACTCCAAGAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D16 (125058)
Gene Description:
TBC1 domain family member 16
Transcript:
RefSeq NM_019020.2 (NON-CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCATCTACTCCAAGAACAA
Hairpin Sequence:
5'-CCGG-GATCATCTACTCCAAGAACAA-CTCGAG-TTGTTCTTGGAGTAGATGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCATCTACTCCAAGAACAACTCGAGTTGTTCTTGGAGTAGATGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCATCTACTCCAAGAACAACTCGAGTTGTTCTTGGAGTAGATGATC-3'

Other clones with same target sequence:

(none)