Construct: shRNA TRCN0000061911

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002274.2-772s1c1
DNA Barcode:
GCCTACATGAAGAAGAACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT13 (3860)
Gene Description:
keratin 13
Transcript:
RefSeq NM_002274.2 (NON-CURRENT)
Match location:
Position 772 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061911)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACATGAAGAAGAACCAT
Hairpin Sequence:
5'-CCGG-GCCTACATGAAGAAGAACCAT-CTCGAG-ATGGTTCTTCTTCATGTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACATGAAGAAGAACCATCTCGAGATGGTTCTTCTTCATGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACATGAAGAAGAACCATCTCGAGATGGTTCTTCTTCATGTAGGC-3'

Other clones with same target sequence:

(none)