Construct: shRNA TRCN0000061918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019081.2-3718s1c1
DNA Barcode:
GCCCAGTTTGTTAAACTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARF1 (9665)
Gene Description:
meiosis regulator and mRNA stability factor 1
Transcript:
RefSeq NM_019081.2 (NON-CURRENT)
Match location:
Position 3718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGTTTGTTAAACTCCTT
Hairpin Sequence:
5'-CCGG-GCCCAGTTTGTTAAACTCCTT-CTCGAG-AAGGAGTTTAACAAACTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGTTTGTTAAACTCCTTCTCGAGAAGGAGTTTAACAAACTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGTTTGTTAAACTCCTTCTCGAGAAGGAGTTTAACAAACTGGGC-3'

Other clones with same target sequence:

(none)