Construct: shRNA TRCN0000062109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004625.3-618s1c1
DNA Barcode:
GCGTTCACCTACGCCATCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WNT7A (7476)
Gene Description:
Wnt family member 7A
Transcript:
RefSeq NM_004625.3 (NON-CURRENT)
Match location:
Position 618 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTCACCTACGCCATCATT
Hairpin Sequence:
5'-CCGG-GCGTTCACCTACGCCATCATT-CTCGAG-AATGATGGCGTAGGTGAACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTCACCTACGCCATCATTCTCGAGAATGATGGCGTAGGTGAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTCACCTACGCCATCATTCTCGAGAATGATGGCGTAGGTGAACGC-3'

Other clones with same target sequence:

(none)