Construct: shRNA TRCN0000062119

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145243.3-1035s1c1
DNA Barcode:
CCGATATTCATCAACTTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OMA1 (115209)
Gene Description:
OMA1 zinc metallopeptidase
Transcript:
RefSeq NM_145243.3 (NON-CURRENT)
Match location:
Position 1035 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062119)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATATTCATCAACTTTCTT
Hairpin Sequence:
5'-CCGG-CCGATATTCATCAACTTTCTT-CTCGAG-AAGAAAGTTGATGAATATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATATTCATCAACTTTCTTCTCGAGAAGAAAGTTGATGAATATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATATTCATCAACTTTCTTCTCGAGAAGAAAGTTGATGAATATCGG-3'

Other clones with same target sequence:

(none)