Construct: shRNA TRCN0000062159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000784.2-444s1c1
DNA Barcode:
GCACCAGTTACAGGTGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP27A1 (1593)
Gene Description:
cytochrome P450 family 27 subfamily A member 1
Transcript:
RefSeq NM_000784.2 (NON-CURRENT)
Match location:
Position 444 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCAGTTACAGGTGCTTTA
Hairpin Sequence:
5'-CCGG-GCACCAGTTACAGGTGCTTTA-CTCGAG-TAAAGCACCTGTAACTGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCAGTTACAGGTGCTTTACTCGAGTAAAGCACCTGTAACTGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCAGTTACAGGTGCTTTACTCGAGTAAAGCACCTGTAACTGGTGC-3'

Other clones with same target sequence:

(none)