Construct: shRNA TRCN0000062329

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018981.1-745s1c1
DNA Barcode:
CCAGTATGAAAGCTGGAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJC10 (54431)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C10
Transcript:
RefSeq NM_018981.1 (NON-CURRENT)
Match location:
Position 745 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062329)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTATGAAAGCTGGAACTA
Hairpin Sequence:
5'-CCGG-CCAGTATGAAAGCTGGAACTA-CTCGAG-TAGTTCCAGCTTTCATACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTATGAAAGCTGGAACTACTCGAGTAGTTCCAGCTTTCATACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTATGAAAGCTGGAACTACTCGAGTAGTTCCAGCTTTCATACTGG-3'

Other clones with same target sequence:

  1. TRCN0000299484