Construct: shRNA TRCN0000062409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002444.2-588s1c1
DNA Barcode:
CCAGTCTAAGTATGGCGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MSN (4478)
Gene Description:
moesin
Transcript:
RefSeq NM_002444.2 (NON-CURRENT)
Match location:
Position 588 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCTAAGTATGGCGACTT
Hairpin Sequence:
5'-CCGG-CCAGTCTAAGTATGGCGACTT-CTCGAG-AAGTCGCCATACTTAGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCTAAGTATGGCGACTTCTCGAGAAGTCGCCATACTTAGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCTAAGTATGGCGACTTCTCGAGAAGTCGCCATACTTAGACTGG-3'

Other clones with same target sequence:

(none)