Construct: shRNA TRCN0000062415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019036.1-846s1c1
DNA Barcode:
CGGCATGGGTTGTTATGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMGCLL1 (54511)
Gene Description:
3-hydroxymethyl-3-methylglutaryl-CoA lyase like 1
Transcript:
RefSeq NM_019036.1 (NON-CURRENT)
Match location:
Position 846 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCATGGGTTGTTATGAGAT
Hairpin Sequence:
5'-CCGG-CGGCATGGGTTGTTATGAGAT-CTCGAG-ATCTCATAACAACCCATGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCATGGGTTGTTATGAGATCTCGAGATCTCATAACAACCCATGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCATGGGTTGTTATGAGATCTCGAGATCTCATAACAACCCATGCCG-3'

Other clones with same target sequence:

(none)