Construct: shRNA TRCN0000062540
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_006444.1-1295s1c1
- DNA Barcode:
- GCCCTTCAAGAAGCAAGTAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SMC2 (10592)
- Gene Description:
- structural maintenance of chromosomes 2
- Transcript:
- RefSeq NM_006444.1 (NON-CURRENT)
- Match location:
- Position 1295 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000062540)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCCTTCAAGAAGCAAGTAAT
- Hairpin Sequence:
-
5'-CCGG-GCCCTTCAAGAAGCAAGTAAT-CTCGAG-ATTACTTGCTTCTTGAAGGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCCTTCAAGAAGCAAGTAATCTCGAGATTACTTGCTTCTTGAAGGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCCTTCAAGAAGCAAGTAATCTCGAGATTACTTGCTTCTTGAAGGGC-3'
Other clones with same target sequence:
-
TRCN0000291365
