Construct: shRNA TRCN0000062545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019043.3-673s1c1
DNA Barcode:
GCCACTGGTATCAGCCAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APBB1IP (54518)
Gene Description:
amyloid beta precursor protein binding family B member 1 interacting protein
Transcript:
RefSeq NM_019043.3 (NON-CURRENT)
Match location:
Position 673 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTGGTATCAGCCAATAT
Hairpin Sequence:
5'-CCGG-GCCACTGGTATCAGCCAATAT-CTCGAG-ATATTGGCTGATACCAGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTGGTATCAGCCAATATCTCGAGATATTGGCTGATACCAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTGGTATCAGCCAATATCTCGAGATATTGGCTGATACCAGTGGC-3'

Other clones with same target sequence:

(none)