Construct: shRNA TRCN0000062577

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001968.2-1310s1c1
DNA Barcode:
CTGTTGTTAATGTTAGAGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF4E (1977)
Gene Description:
eukaryotic translation initiation factor 4E
Transcript:
RefSeq NM_001968.2 (NON-CURRENT)
Match location:
Position 1310 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062577)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTGTTAATGTTAGAGCTA
Hairpin Sequence:
5'-CCGG-CTGTTGTTAATGTTAGAGCTA-CTCGAG-TAGCTCTAACATTAACAACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTGTTAATGTTAGAGCTACTCGAGTAGCTCTAACATTAACAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTGTTAATGTTAGAGCTACTCGAGTAGCTCTAACATTAACAACAG-3'

Other clones with same target sequence:

(none)