Construct: shRNA TRCN0000062666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018963.3-2575s1c1
DNA Barcode:
GCGTGAGTTATCTTCTGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRWD1 (54014)
Gene Description:
bromodomain and WD repeat domain containing 1
Transcript:
RefSeq NM_018963.3 (NON-CURRENT)
Match location:
Position 2575 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGAGTTATCTTCTGGAAA
Hairpin Sequence:
5'-CCGG-GCGTGAGTTATCTTCTGGAAA-CTCGAG-TTTCCAGAAGATAACTCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGAGTTATCTTCTGGAAACTCGAGTTTCCAGAAGATAACTCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGAGTTATCTTCTGGAAACTCGAGTTTCCAGAAGATAACTCACGC-3'

Other clones with same target sequence:

(none)