Construct: shRNA TRCN0000062688

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018991.2-1377s1c1
DNA Barcode:
GCCCAGCATGTTAGACAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAG3L1 (54441)
Gene Description:
stromal antigen 3-like 1 (pseudogene)
Transcript:
RefSeq NM_018991.2 (NON-CURRENT)
Match location:
Position 1377 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062688)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGCATGTTAGACAATTT
Hairpin Sequence:
5'-CCGG-GCCCAGCATGTTAGACAATTT-CTCGAG-AAATTGTCTAACATGCTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGCATGTTAGACAATTTCTCGAGAAATTGTCTAACATGCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGCATGTTAGACAATTTCTCGAGAAATTGTCTAACATGCTGGGC-3'

Other clones with same target sequence:

(none)