Construct: shRNA TRCN0000062730

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004747.2-2175s1c1
DNA Barcode:
CCAGGCTCATAACAAACGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DLG5 (9231)
Gene Description:
discs large MAGUK scaffold protein 5
Transcript:
RefSeq NM_004747.2 (NON-CURRENT)
Match location:
Position 2175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062730)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCTCATAACAAACGGAA
Hairpin Sequence:
5'-CCGG-CCAGGCTCATAACAAACGGAA-CTCGAG-TTCCGTTTGTTATGAGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCTCATAACAAACGGAACTCGAGTTCCGTTTGTTATGAGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCTCATAACAAACGGAACTCGAGTTCCGTTTGTTATGAGCCTGG-3'

Other clones with same target sequence:

(none)