Construct: shRNA TRCN0000062751

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018330.3-970s1c1
DNA Barcode:
CGAAAGAAAGCAGAGTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHTN1 (57698)
Gene Description:
shootin 1
Transcript:
RefSeq NM_018330.3 (NON-CURRENT)
Match location:
Position 970 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062751)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGAAAGCAGAGTCATTT
Hairpin Sequence:
5'-CCGG-CGAAAGAAAGCAGAGTCATTT-CTCGAG-AAATGACTCTGCTTTCTTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGAAAGCAGAGTCATTTCTCGAGAAATGACTCTGCTTTCTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGAAAGCAGAGTCATTTCTCGAGAAATGACTCTGCTTTCTTTCG-3'

Other clones with same target sequence:

  1. TRCN0000286928