Construct: shRNA TRCN0000062753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015488.3-2210s1c1
DNA Barcode:
CCAGCCATATTCCCATGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PNKD (25953)
Gene Description:
PNKD metallo-beta-lactamase domain containing
Transcript:
RefSeq NM_015488.3 (NON-CURRENT)
Match location:
Position 2210 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCATATTCCCATGTGAT
Hairpin Sequence:
5'-CCGG-CCAGCCATATTCCCATGTGAT-CTCGAG-ATCACATGGGAATATGGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCATATTCCCATGTGATCTCGAGATCACATGGGAATATGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCATATTCCCATGTGATCTCGAGATCACATGGGAATATGGCTGG-3'

Other clones with same target sequence:

(none)