Construct: shRNA TRCN0000062794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019054.1-1497s1c1
DNA Barcode:
CGGACCAAATTCTGGCTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHLD2 (54537)
Gene Description:
shieldin complex subunit 2
Transcript:
RefSeq NM_019054.1 (NON-CURRENT)
Match location:
Position 1497 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACCAAATTCTGGCTCTAA
Hairpin Sequence:
5'-CCGG-CGGACCAAATTCTGGCTCTAA-CTCGAG-TTAGAGCCAGAATTTGGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACCAAATTCTGGCTCTAACTCGAGTTAGAGCCAGAATTTGGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACCAAATTCTGGCTCTAACTCGAGTTAGAGCCAGAATTTGGTCCG-3'

Other clones with same target sequence:

(none)