Construct: shRNA TRCN0000062919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017705.2-1242s1c1
DNA Barcode:
GCCCTTCTCTTTCTCTCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAQR5 (54852)
Gene Description:
progestin and adipoQ receptor family member 5
Transcript:
RefSeq NM_017705.2 (NON-CURRENT)
Match location:
Position 1242 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTCTCTTTCTCTCAGAT
Hairpin Sequence:
5'-CCGG-GCCCTTCTCTTTCTCTCAGAT-CTCGAG-ATCTGAGAGAAAGAGAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTCTCTTTCTCTCAGATCTCGAGATCTGAGAGAAAGAGAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTCTCTTTCTCTCAGATCTCGAGATCTGAGAGAAAGAGAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000333779