Construct: shRNA TRCN0000062940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014294.3-1034s1c1
DNA Barcode:
ACTGGAAACTTCAATGTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAM1 (23471)
Gene Description:
translocation associated membrane protein 1
Transcript:
RefSeq NM_014294.3 (NON-CURRENT)
Match location:
Position 1034 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000062940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGAAACTTCAATGTGTTA
Hairpin Sequence:
5'-CCGG-ACTGGAAACTTCAATGTGTTA-CTCGAG-TAACACATTGAAGTTTCCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGAAACTTCAATGTGTTACTCGAGTAACACATTGAAGTTTCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGAAACTTCAATGTGTTACTCGAGTAACACATTGAAGTTTCCAGT-3'

Other clones with same target sequence:

  1. TRCN0000300789