Construct: shRNA TRCN0000063011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018010.2-1189s1c1
DNA Barcode:
GCTCCTTTGGTGAAGATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IFT57 (55081)
Gene Description:
intraflagellar transport 57
Transcript:
RefSeq NM_018010.2 (NON-CURRENT)
Match location:
Position 1189 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCTTTGGTGAAGATTAAA
Hairpin Sequence:
5'-CCGG-GCTCCTTTGGTGAAGATTAAA-CTCGAG-TTTAATCTTCACCAAAGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCTTTGGTGAAGATTAAACTCGAGTTTAATCTTCACCAAAGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCTTTGGTGAAGATTAAACTCGAGTTTAATCTTCACCAAAGGAGC-3'

Other clones with same target sequence:

  1. TRCN0000299280