Construct: shRNA TRCN0000063025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006737.1-570s1c1
DNA Barcode:
GCAGGAACCTACAGATGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIR3DL2 (3812)
Gene Description:
killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 2
Transcript:
RefSeq NM_006737.1 (NON-CURRENT)
Match location:
Position 570 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAACCTACAGATGTTAT
Hairpin Sequence:
5'-CCGG-GCAGGAACCTACAGATGTTAT-CTCGAG-ATAACATCTGTAGGTTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAACCTACAGATGTTATCTCGAGATAACATCTGTAGGTTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAACCTACAGATGTTATCTCGAGATAACATCTGTAGGTTCCTGC-3'

Other clones with same target sequence:

(none)