Construct: shRNA TRCN0000063041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052939.2-754s1c1
DNA Barcode:
CTCCAGGGATAATAGCAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FCRL3 (115352)
Gene Description:
Fc receptor like 3
Transcript:
RefSeq NM_052939.2 (NON-CURRENT)
Match location:
Position 754 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAGGGATAATAGCAAATA
Hairpin Sequence:
5'-CCGG-CTCCAGGGATAATAGCAAATA-CTCGAG-TATTTGCTATTATCCCTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAGGGATAATAGCAAATACTCGAGTATTTGCTATTATCCCTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAGGGATAATAGCAAATACTCGAGTATTTGCTATTATCCCTGGAG-3'

Other clones with same target sequence:

(none)