Construct: shRNA TRCN0000063070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001510.1-906s1c1
DNA Barcode:
CCATCGAATATCTTCAACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRID2 (2895)
Gene Description:
glutamate ionotropic receptor delta type subunit 2
Transcript:
RefSeq NM_001510.1 (NON-CURRENT)
Match location:
Position 906 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this shRNA:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 human 2895 GRID2 glutamate ionotropic recept... NM_001286838.1 100% CDS 879 5.625 4.500
2 human 2895 GRID2 glutamate ionotropic recept... NM_001510.4 100% CDS 1597 5.625 4.500
3 human 2895 GRID2 glutamate ionotropic recept... XM_011531893.2 100% CDS 7353 5.625 4.500
4 human 2895 GRID2 glutamate ionotropic recept... XM_011531894.2 100% CDS 392 5.625 4.500
5 human 2895 GRID2 glutamate ionotropic recept... XM_011531895.2 100% 5UTR 309 5.625 4.500
6 human 2895 GRID2 glutamate ionotropic recept... XM_017008118.1 100% CDS 7353 5.625 4.500
7 human 2895 GRID2 glutamate ionotropic recept... XM_017008119.1 100% CDS 890 5.625 4.500
8 human 2895 GRID2 glutamate ionotropic recept... XM_017008120.2 100% CDS 7353 5.625 4.500
9 human 2895 GRID2 glutamate ionotropic recept... XM_017008121.1 100% CDS 7353 5.625 4.500
10 human 2895 GRID2 glutamate ionotropic recept... XM_017008122.2 100% CDS 1941 5.625 4.500
11 human 2895 GRID2 glutamate ionotropic recept... XM_017008123.1 100% 5UTR 1668 5.625 4.500
12 human 2895 GRID2 glutamate ionotropic recept... XM_017008124.1 100% 5UTR 888 5.625 4.500
13 human 2895 GRID2 glutamate ionotropic recept... XM_017008125.1 100% 5UTR 385 5.625 4.500
14 human 2895 GRID2 glutamate ionotropic recept... XM_017008127.1 100% CDS 7353 5.625 4.500
15 human 2895 GRID2 glutamate ionotropic recept... XM_024454024.1 100% CDS 1941 5.625 4.500
16 human 2895 GRID2 glutamate ionotropic recept... XM_024454025.1 100% 5UTR 309 5.625 4.500
17 human 2895 GRID2 glutamate ionotropic recept... XM_024454026.1 100% 5UTR 320 5.625 4.500
18 human 100130557 NFYC-AS1 NFYC antisense RNA 1 NR_024567.1 85% 3UTR 1320
19 mouse 14804 Grid2 glutamate receptor, ionotro... NM_008167.2 89% CDS 906
20 mouse 14804 Grid2 glutamate receptor, ionotro... XM_006505571.3 89% CDS 1860
21 mouse 70829 Ccdc93 coiled-coil domain containi... NM_001025156.2 85% 3UTR 4821
22 mouse 70829 Ccdc93 coiled-coil domain containi... NM_029955.3 85% 3UTR 4818
23 mouse 385643 Kng2 kininogen 2 NM_001102409.1 95% CDS 677
24 mouse 385643 Kng2 kininogen 2 NM_001102410.1 95% CDS 677
25 mouse 385643 Kng2 kininogen 2 NM_201375.2 95% CDS 677
26 mouse 385643 Kng2 kininogen 2 XM_006522324.1 95% CDS 365
27 mouse 320586 A630089N07Rik RIKEN cDNA A630089N07 gene NR_015491.1 89% 3UTR 1330
28 mouse 63830 Kcnq1ot1 KCNQ1 overlapping transcript 1 NR_001461.5 89% 3UTR 67511
29 mouse 105244262 Gm39903 predicted gene, 39903 XR_866637.2 89% 3UTR 1797
Download CSV

Sequence Information

Target Sequence:
CCATCGAATATCTTCAACATT
Hairpin Sequence:
5'-CCGG-CCATCGAATATCTTCAACATT-CTCGAG-AATGTTGAAGATATTCGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCGAATATCTTCAACATTCTCGAGAATGTTGAAGATATTCGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCGAATATCTTCAACATTCTCGAGAATGTTGAAGATATTCGATGG-3'

Other clones with same target sequence:

(none)