Construct: shRNA TRCN0000063085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016011.1-807s1c1
DNA Barcode:
CTCAACTGTGTTGGTGGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MECR (51102)
Gene Description:
mitochondrial trans-2-enoyl-CoA reductase
Transcript:
RefSeq NM_016011.1 (NON-CURRENT)
Match location:
Position 807 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAACTGTGTTGGTGGGAAA
Hairpin Sequence:
5'-CCGG-CTCAACTGTGTTGGTGGGAAA-CTCGAG-TTTCCCACCAACACAGTTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAACTGTGTTGGTGGGAAACTCGAGTTTCCCACCAACACAGTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAACTGTGTTGGTGGGAAACTCGAGTTTCCCACCAACACAGTTGAG-3'

Other clones with same target sequence:

(none)