Construct: shRNA TRCN0000063113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153443.2-756s1c1
DNA Barcode:
CCGGAGCTTGTTTGACATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIR3DL3 (115653)
Gene Description:
killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 3
Transcript:
RefSeq NM_153443.2 (NON-CURRENT)
Match location:
Position 756 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGCTTGTTTGACATTTA
Hairpin Sequence:
5'-CCGG-CCGGAGCTTGTTTGACATTTA-CTCGAG-TAAATGTCAAACAAGCTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGCTTGTTTGACATTTACTCGAGTAAATGTCAAACAAGCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGCTTGTTTGACATTTACTCGAGTAAATGTCAAACAAGCTCCGG-3'

Other clones with same target sequence:

(none)