Construct: shRNA TRCN0000063164

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000856.1-1079s1c1
DNA Barcode:
CCTCCATTCTATGCCTGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUCY1A1 (2982)
Gene Description:
guanylate cyclase 1 soluble subunit alpha 1
Transcript:
RefSeq NM_000856.1 (NON-CURRENT)
Match location:
Position 1079 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063164)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCATTCTATGCCTGGATA
Hairpin Sequence:
5'-CCGG-CCTCCATTCTATGCCTGGATA-CTCGAG-TATCCAGGCATAGAATGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCATTCTATGCCTGGATACTCGAGTATCCAGGCATAGAATGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCATTCTATGCCTGGATACTCGAGTATCCAGGCATAGAATGGAGG-3'

Other clones with same target sequence:

(none)