Construct: shRNA TRCN0000063201

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021956.2-1486s1c1
DNA Barcode:
CCAATCGTTCTTTGATTGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIK2 (2898)
Gene Description:
glutamate ionotropic receptor kainate type subunit 2
Transcript:
RefSeq NM_021956.2 (NON-CURRENT)
Match location:
Position 1486 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063201)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATCGTTCTTTGATTGTTA
Hairpin Sequence:
5'-CCGG-CCAATCGTTCTTTGATTGTTA-CTCGAG-TAACAATCAAAGAACGATTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATCGTTCTTTGATTGTTACTCGAGTAACAATCAAAGAACGATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATCGTTCTTTGATTGTTACTCGAGTAACAATCAAAGAACGATTGG-3'

Other clones with same target sequence:

(none)