Construct: shRNA TRCN0000063210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020858.1-792s1c1
DNA Barcode:
GCCACAACTTTATCAAAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEMA6D (80031)
Gene Description:
semaphorin 6D
Transcript:
RefSeq NM_020858.1 (NON-CURRENT)
Match location:
Position 792 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACAACTTTATCAAAGTAT
Hairpin Sequence:
5'-CCGG-GCCACAACTTTATCAAAGTAT-CTCGAG-ATACTTTGATAAAGTTGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACAACTTTATCAAAGTATCTCGAGATACTTTGATAAAGTTGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACAACTTTATCAAAGTATCTCGAGATACTTTGATAAAGTTGTGGC-3'

Other clones with same target sequence:

(none)