Construct: shRNA TRCN0000063215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152754.2-1716s1c1
DNA Barcode:
GCTCTCGATATGCTCCTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEMA3D (223117)
Gene Description:
semaphorin 3D
Transcript:
RefSeq NM_152754.2 (CURRENT)
Match location:
Position 1716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCGATATGCTCCTACTT
Hairpin Sequence:
5'-CCGG-GCTCTCGATATGCTCCTACTT-CTCGAG-AAGTAGGAGCATATCGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCGATATGCTCCTACTTCTCGAGAAGTAGGAGCATATCGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCGATATGCTCCTACTTCTCGAGAAGTAGGAGCATATCGAGAGC-3'

Other clones with same target sequence:

(none)