Construct: shRNA TRCN0000063315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014619.2-1568s1c1
DNA Barcode:
GCATTCTTTACCGCGTTCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIK4 (2900)
Gene Description:
glutamate ionotropic receptor kainate type subunit 4
Transcript:
RefSeq NM_014619.2 (NON-CURRENT)
Match location:
Position 1568 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCTTTACCGCGTTCATA
Hairpin Sequence:
5'-CCGG-GCATTCTTTACCGCGTTCATA-CTCGAG-TATGAACGCGGTAAAGAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCTTTACCGCGTTCATACTCGAGTATGAACGCGGTAAAGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCTTTACCGCGTTCATACTCGAGTATGAACGCGGTAAAGAATGC-3'

Other clones with same target sequence:

(none)