Construct: shRNA TRCN0000063328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002088.3-2198s1c1
DNA Barcode:
CGAGTCCACCATGAACGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIK5 (2901)
Gene Description:
glutamate ionotropic receptor kainate type subunit 5
Transcript:
RefSeq NM_002088.3 (NON-CURRENT)
Match location:
Position 2198 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTCCACCATGAACGAATA
Hairpin Sequence:
5'-CCGG-CGAGTCCACCATGAACGAATA-CTCGAG-TATTCGTTCATGGTGGACTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTCCACCATGAACGAATACTCGAGTATTCGTTCATGGTGGACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTCCACCATGAACGAATACTCGAGTATTCGTTCATGGTGGACTCG-3'

Other clones with same target sequence:

(none)