Construct: shRNA TRCN0000063371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000832.4-2137s1c1
DNA Barcode:
CGCCAACTACAGCATCATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIN1 (2902)
Gene Description:
glutamate ionotropic receptor NMDA type subunit 1
Transcript:
RefSeq NM_000832.4 (NON-CURRENT)
Match location:
Position 2137 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAACTACAGCATCATGAA
Hairpin Sequence:
5'-CCGG-CGCCAACTACAGCATCATGAA-CTCGAG-TTCATGATGCTGTAGTTGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAACTACAGCATCATGAACTCGAGTTCATGATGCTGTAGTTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAACTACAGCATCATGAACTCGAGTTCATGATGCTGTAGTTGGCG-3'

Other clones with same target sequence:

(none)