Construct: shRNA TRCN0000063416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058172.1-578s1c1
DNA Barcode:
GCACTTACACTGTAAATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANTXR2 (118429)
Gene Description:
ANTXR cell adhesion molecule 2
Transcript:
RefSeq NM_058172.1 (NON-CURRENT)
Match location:
Position 578 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTACACTGTAAATGAAA
Hairpin Sequence:
5'-CCGG-GCACTTACACTGTAAATGAAA-CTCGAG-TTTCATTTACAGTGTAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTACACTGTAAATGAAACTCGAGTTTCATTTACAGTGTAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTACACTGTAAATGAAACTCGAGTTTCATTTACAGTGTAAGTGC-3'

Other clones with same target sequence:

(none)