Construct: shRNA TRCN0000063438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207370.1-1397s1c1
DNA Barcode:
GCAGGAGGACAAGATGCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPR153 (387509)
Gene Description:
G protein-coupled receptor 153
Transcript:
RefSeq NM_207370.1 (NON-CURRENT)
Match location:
Position 1397 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAGGACAAGATGCAATA
Hairpin Sequence:
5'-CCGG-GCAGGAGGACAAGATGCAATA-CTCGAG-TATTGCATCTTGTCCTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAGGACAAGATGCAATACTCGAGTATTGCATCTTGTCCTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAGGACAAGATGCAATACTCGAGTATTGCATCTTGTCCTCCTGC-3'

Other clones with same target sequence:

(none)