Construct: shRNA TRCN0000063485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000833.2-3218s1c1
DNA Barcode:
CGGCAGAAGGATAACCTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIN2A (2903)
Gene Description:
glutamate ionotropic receptor NMDA type subunit 2A
Transcript:
RefSeq NM_000833.2 (NON-CURRENT)
Match location:
Position 3218 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAGAAGGATAACCTCAAT
Hairpin Sequence:
5'-CCGG-CGGCAGAAGGATAACCTCAAT-CTCGAG-ATTGAGGTTATCCTTCTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAGAAGGATAACCTCAATCTCGAGATTGAGGTTATCCTTCTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAGAAGGATAACCTCAATCTCGAGATTGAGGTTATCCTTCTGCCG-3'

Other clones with same target sequence:

(none)