Construct: shRNA TRCN0000063679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000812.2-981s1c1
DNA Barcode:
GCCAAAGATCCCTTATGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRB1 (2560)
Gene Description:
gamma-aminobutyric acid type A receptor beta1 subunit
Transcript:
RefSeq NM_000812.2 (NON-CURRENT)
Match location:
Position 981 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAAGATCCCTTATGTCAA
Hairpin Sequence:
5'-CCGG-GCCAAAGATCCCTTATGTCAA-CTCGAG-TTGACATAAGGGATCTTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAAGATCCCTTATGTCAACTCGAGTTGACATAAGGGATCTTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAAGATCCCTTATGTCAACTCGAGTTGACATAAGGGATCTTTGGC-3'

Other clones with same target sequence:

(none)