Construct: shRNA TRCN0000063702

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006989.3-1879s1c1
DNA Barcode:
CCTCATCAAGTTAGCCAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASA4 (10156)
Gene Description:
RAS p21 protein activator 4
Transcript:
RefSeq NM_006989.3 (NON-CURRENT)
Match location:
Position 1879 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063702)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATCAAGTTAGCCAACAT
Hairpin Sequence:
5'-CCGG-CCTCATCAAGTTAGCCAACAT-CTCGAG-ATGTTGGCTAACTTGATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATCAAGTTAGCCAACATCTCGAGATGTTGGCTAACTTGATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATCAAGTTAGCCAACATCTCGAGATGTTGGCTAACTTGATGAGG-3'

Other clones with same target sequence:

(none)