Construct: shRNA TRCN0000063734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004536.1-3718s1c1
DNA Barcode:
GCTGAGTATGATCCTTCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAIP (4671)
Gene Description:
NLR family apoptosis inhibitory protein
Transcript:
RefSeq NM_004536.1 (NON-CURRENT)
Match location:
Position 3718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGTATGATCCTTCCAAA
Hairpin Sequence:
5'-CCGG-GCTGAGTATGATCCTTCCAAA-CTCGAG-TTTGGAAGGATCATACTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGTATGATCCTTCCAAACTCGAGTTTGGAAGGATCATACTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGTATGATCCTTCCAAACTCGAGTTTGGAAGGATCATACTCAGC-3'

Other clones with same target sequence:

(none)