Construct: shRNA TRCN0000063843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006005.2-1397s1c1
DNA Barcode:
GCTCTCTGTCTTCTTCGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WFS1 (7466)
Gene Description:
wolframin ER transmembrane glycoprotein
Transcript:
RefSeq NM_006005.2 (NON-CURRENT)
Match location:
Position 1397 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCTGTCTTCTTCGTCAT
Hairpin Sequence:
5'-CCGG-GCTCTCTGTCTTCTTCGTCAT-CTCGAG-ATGACGAAGAAGACAGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCTGTCTTCTTCGTCATCTCGAGATGACGAAGAAGACAGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCTGTCTTCTTCGTCATCTCGAGATGACGAAGAAGACAGAGAGC-3'

Other clones with same target sequence:

(none)