Construct: shRNA TRCN0000063861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016441.1-1868s1c1
DNA Barcode:
CCGTGGATGGTCATCATCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRIM1 (51232)
Gene Description:
cysteine rich transmembrane BMP regulator 1
Transcript:
RefSeq NM_016441.1 (NON-CURRENT)
Match location:
Position 1868 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGGATGGTCATCATCATA
Hairpin Sequence:
5'-CCGG-CCGTGGATGGTCATCATCATA-CTCGAG-TATGATGATGACCATCCACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGGATGGTCATCATCATACTCGAGTATGATGATGACCATCCACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGGATGGTCATCATCATACTCGAGTATGATGATGACCATCCACGG-3'

Other clones with same target sequence:

  1. TRCN0000289444