Construct: shRNA TRCN0000063875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005503.2-1399s1c1
DNA Barcode:
GCCGGGTCAAGAGGATGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APBA2 (321)
Gene Description:
amyloid beta precursor protein binding family A member 2
Transcript:
RefSeq NM_005503.2 (NON-CURRENT)
Match location:
Position 1399 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGGTCAAGAGGATGCAAA
Hairpin Sequence:
5'-CCGG-GCCGGGTCAAGAGGATGCAAA-CTCGAG-TTTGCATCCTCTTGACCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGGTCAAGAGGATGCAAACTCGAGTTTGCATCCTCTTGACCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGGTCAAGAGGATGCAAACTCGAGTTTGCATCCTCTTGACCCGGC-3'

Other clones with same target sequence:

(none)