Construct: shRNA TRCN0000063893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018717.2-2483s1c1
DNA Barcode:
GCCAAATAACTTGGGTACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAML3 (55534)
Gene Description:
mastermind like transcriptional coactivator 3
Transcript:
RefSeq NM_018717.2 (NON-CURRENT)
Match location:
Position 2483 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAATAACTTGGGTACAAA
Hairpin Sequence:
5'-CCGG-GCCAAATAACTTGGGTACAAA-CTCGAG-TTTGTACCCAAGTTATTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAATAACTTGGGTACAAACTCGAGTTTGTACCCAAGTTATTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAATAACTTGGGTACAAACTCGAGTTTGTACCCAAGTTATTTGGC-3'

Other clones with same target sequence:

(none)