Construct: shRNA TRCN0000063910

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004352.1-542s1c1
DNA Barcode:
GATGAGTAATCGCACCATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBLN1 (869)
Gene Description:
cerebellin 1 precursor
Transcript:
RefSeq NM_004352.1 (NON-CURRENT)
Match location:
Position 542 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063910)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGTAATCGCACCATGAT
Hairpin Sequence:
5'-CCGG-GATGAGTAATCGCACCATGAT-CTCGAG-ATCATGGTGCGATTACTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGTAATCGCACCATGATCTCGAGATCATGGTGCGATTACTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGTAATCGCACCATGATCTCGAGATCATGGTGCGATTACTCATC-3'

Other clones with same target sequence:

(none)