Construct: shRNA TRCN0000063952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021080.3-2264s1c1
DNA Barcode:
CTCATCCAACAGTGATCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAB1 (1600)
Gene Description:
DAB adaptor protein 1
Transcript:
RefSeq NM_021080.3 (NON-CURRENT)
Match location:
Position 2264 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATCCAACAGTGATCCATT
Hairpin Sequence:
5'-CCGG-CTCATCCAACAGTGATCCATT-CTCGAG-AATGGATCACTGTTGGATGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATCCAACAGTGATCCATTCTCGAGAATGGATCACTGTTGGATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATCCAACAGTGATCCATTCTCGAGAATGGATCACTGTTGGATGAG-3'

Other clones with same target sequence:

(none)