Construct: shRNA TRCN0000063978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004085.2-286s1c1
DNA Barcode:
CCCAGAAATCCAAGCCAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIMM8A (1678)
Gene Description:
translocase of inner mitochondrial membrane 8A
Transcript:
RefSeq NM_004085.2 (NON-CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAAATCCAAGCCAGTTT
Hairpin Sequence:
5'-CCGG-CCCAGAAATCCAAGCCAGTTT-CTCGAG-AAACTGGCTTGGATTTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAAATCCAAGCCAGTTTCTCGAGAAACTGGCTTGGATTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAAATCCAAGCCAGTTTCTCGAGAAACTGGCTTGGATTTCTGGG-3'

Other clones with same target sequence:

(none)