Construct: shRNA TRCN0000064030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000766.3-51s1c1
DNA Barcode:
CCTGACTGTGATGGTCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP2A13 (1553)
Gene Description:
cytochrome P450 family 2 subfamily A member 13
Transcript:
RefSeq NM_000766.3 (NON-CURRENT)
Match location:
Position 51 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACTGTGATGGTCTTGAT
Hairpin Sequence:
5'-CCGG-CCTGACTGTGATGGTCTTGAT-CTCGAG-ATCAAGACCATCACAGTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACTGTGATGGTCTTGATCTCGAGATCAAGACCATCACAGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACTGTGATGGTCTTGATCTCGAGATCAAGACCATCACAGTCAGG-3'

Other clones with same target sequence:

(none)