Construct: shRNA TRCN0000064060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000769.1-1225s1c1
DNA Barcode:
CCTCGTCACTTTCTGGATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP2C19 (1557)
Gene Description:
cytochrome P450 family 2 subfamily C member 19
Transcript:
RefSeq NM_000769.1 (NON-CURRENT)
Match location:
Position 1225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGTCACTTTCTGGATGAA
Hairpin Sequence:
5'-CCGG-CCTCGTCACTTTCTGGATGAA-CTCGAG-TTCATCCAGAAAGTGACGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGTCACTTTCTGGATGAACTCGAGTTCATCCAGAAAGTGACGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGTCACTTTCTGGATGAACTCGAGTTCATCCAGAAAGTGACGAGG-3'

Other clones with same target sequence:

(none)