Construct: shRNA TRCN0000064172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_051699.4-1900s1c1
DNA Barcode:
CCAGCTCAACAGGATTTCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TXNDC16 (57544)
Gene Description:
thioredoxin domain containing 16
Transcript:
RefSeq XM_051699.4 (NON-CURRENT)
Match location:
Position 1900 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTCAACAGGATTTCATA
Hairpin Sequence:
5'-CCGG-CCAGCTCAACAGGATTTCATA-CTCGAG-TATGAAATCCTGTTGAGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTCAACAGGATTTCATACTCGAGTATGAAATCCTGTTGAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTCAACAGGATTTCATACTCGAGTATGAAATCCTGTTGAGCTGG-3'

Other clones with same target sequence:

(none)