Construct: shRNA TRCN0000064253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022820.3-1348s1c1
DNA Barcode:
CCCTGAAAGTAGGTTCAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP3A43 (64816)
Gene Description:
cytochrome P450 family 3 subfamily A member 43
Transcript:
RefSeq NM_022820.3 (NON-CURRENT)
Match location:
Position 1348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000064253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGAAAGTAGGTTCAGTAA
Hairpin Sequence:
5'-CCGG-CCCTGAAAGTAGGTTCAGTAA-CTCGAG-TTACTGAACCTACTTTCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGAAAGTAGGTTCAGTAACTCGAGTTACTGAACCTACTTTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGAAAGTAGGTTCAGTAACTCGAGTTACTGAACCTACTTTCAGGG-3'

Other clones with same target sequence:

(none)